VitaScript™ FirstStrand cDNA Synthesis Kit (50 rxn)
VitaScript™ FirstStrand cDNA Synthesis Kit is a set of reagents for transcription of RNA template into cDNA. This step is often found in gene expression analysis where whole RNA pools are transcribed and then subsequently analyzed in quantitative PCR.
Performing reverse transcription in a separate step is recommended if
- you want high flexibility in your reaction setup
- you want to analyze rare targets: the use of random primers increases template coverage
- you want high sensitivity: longer reverse transcription protocols for a more complete coverage of the RNA pool
- you want to store the cDNA library for further studies
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