When selecting a universal PCR master mix, the biggest pitfalls to avoid include:
- Incompatible reaction conditions: Some master mixes may not be suitable for certain types of PCR, such as high-throughput or long-amplicon PCR, due to differences in reaction conditions.
- Low specificity or sensitivity: The mix may not be able to detect or amplify a specific target or may produce false positives, resulting in inaccurate results.
- Inadequate storage conditions: Some master mixes may be sensitive to temperature or light, and may degrade if stored improperly.
- Poor performance with certain template types: Some master mixes may not perform well with certain template types, such as GC-rich or AT-rich sequences, resulting in low amplification efficiency.
- High cost: some master mixes can be expensive which can affect the number of experiments that can be done in your budget.
Lämna en kommentar
Denna webbplats är skyddad av reCAPTCHA och Googles integritetspolicy . Användarvillkor gäller.