NGS Service
We offer a full range of Next Generation Sequencing services, including nucleic acid extraction, library preparation, sequencing and bioinformatic analysis.
Trax Lab Systems is at the forefront of NGS. We utilize high fidelity polymerases used for Next Generation Sequencing pipelines and custom made kits produced in our biotech partner's facility. We use the most advanced technologies available and strive for continuous development. This enables us to be quick, efficient and affordable.
Our services are run by experienced staff who are easily contactable via phone or email. We are available to give advice at any stage of your project and we can assist you in finding a suitable sequencing solution. Our mission is to be the most comprehensive supplier of NextGen services, offering a range of speeds and outputs.
DNA Sequencing
- Whole Exome Sequencing
- Whole Genome Sequencing
- Whole Genome Bisulphite Sequencing
- Shotgun Metagenome Analysis
WGS/WGBS Specifications
Whole genome sequencing (WGS) provides the most comprehensive analysis of genome variance and structure. WGS enables accurate detection of single nucleotide and structural variations of human genomes.
Whole genome bisulfite sequencing (WGBS) reveals differentially methylated sites, which can be visualized on the genome at single-nucleotide resolution.
Trax Lab Systems offers WGS/WGBS services for accurate variant detection using the Illumina NovaSeq 6000 and Dragen Bio-IT platforms.
Sample
≥20 µl, ≥50 ng/µl High quality DNA
Library preparation
PerkinElmer/NEB/Zymo
Inclusions
- Sequencing
- Bioinformatics
- Raw data transfer
WGS analysis includes sample quality control, automated library preparation, final QC, sequencing and full bioinformatic analysis* (filtering, trimming, mapping, annotation and variant reports); free data transfer of FastQ, Bam files and Reports
Sequencing metrics
Performed on Illumina NovaSeq 6000 with 2×150 bp paired-end run; Seq throughput: 30x coverage, based on genome size (human: 90 Gbp read per sample); Q30 ≥ 80%
Quality control
Full quality assurance and guaranteed specifications
Turnaround time
min. 2 – max. 4 weeks
Prices
For our best prices please contact us at info@traxconnects.com or call us at 833-548-8378.
RNA Sequencing
- Gene Expression Profiling
- mRNA-Sequencing
- RNA-Sequencing
- Small RNA Sequencing
GEx Specifications
- Gene Expression Profing (GEx): determines the gene-level abundance and differential expression of coding transcripts.
- mRNA-Seq : targeting poly(A) RNAs provides a sensitive option for detection and quantitation of even rare coding transcript variants.
- RNA-Seq : RNA samples are enriched for both mRNA and non-coding RNAs to provide a comprehensive view of the whole transcriptome.
- Small RNA-Seq : targets small regulatory RNAs such as miRNA for profiling and discovery.
Sample
≥20 µl, ≥25 ng/µl High or Low quality RNA
Library Preparation
QuantSeq 3’ mRNA-Seq Library Prep Kit (Lexogen)
Inclusions
- Sequencing
- Bioinformatics
- Raw data transfer
GEx analysis includes sample quality control, automated library preparation, final QC, sequencing and full bioinformatic analysis (filtering, trimming, mapping, annotation, differential expression and clinical reports); free data transfer of FastQ, Bam files and Reports
Sequencing metrics
Performed on Illumina NextSeq/NovaSeq with 1x≥50 bp single-end run; Seq throughput: 10/20 M SE read per sample; Q30 ≥ 85%
Quality control
Full quality assurance and guaranteed specifications
Turnaround time
min. 2 – max. 4 weeks
Prices
For our best prices please contact us at info@traxconnects.com or call us at 833-548-8378.
Trax Lab Systems Custom Solutions and Kits
We collaborate to create our own custom kits, enabling us to offer quick and affordable analysis solutions.
See our custom solutions below.
Covid Sequencing
- A multiplexed amplicon-based whole-viral-genome sequencing approach.
- Trax Lab Systems' PT-CoVSeq pipeline is designed for targeted NGS library preparation starting from SARS-CoV-2 RNA.
- The assay enables medium to high throughput sequencing of complete viral genomes and allows mutational analysis and variant detection.
- It combines the open source ARTIC v3 primer set with the company’s proprietary enzymes and fine-tuned buffers for the strand syntheses and indexing PCR steps.
Our other NGS Services
- Targeted Sequencing
- ChIP-Seq
- Amplicon Sequencing
- Metagenomics
- MethylSeq
- Immune Repertoire Analysis
- Bioinformatics & Biostatistics
Trax Lab Systems NGS
Portfolio
We offer a full range of Next Generation Sequencing services, including nucleic acid extraction, library preparation, sequencing and bioinformatic analysis.
Mission
Our mission is to be the most comprehensive supplier of NextGen services, offering a range of speeds and outputs.
Custom Solutions
Our services are run by experienced staff who are easily contactable via phone or email. We are available to give advice at any stage of your project and we can assist you in finding a suitable sequencing solution.
Quality Policy
As dedicated scientists, we know that every experiment is invaluable. High quality and replication are the backbone for every analytical test and secure transfer and storage of samples.